Histamine

Histamine, b-imidazolethylamine is an intercellular mediator exerting potent effects on various target tissues. Histamine is stored in large amounts in metachromatic granules of mast cells and basophils, and it is one of the major mediators of immediate hypersensitivity. It is released actively from mast cells and basophils when challenged with allergens to which the allergic patient is sensitized or with non-specific histamine releasers such as IgE or pharmacological agents. Histamine is also an important mediator in the central nervous system and the gastric mucosa. The determination of histamine can be used for the aid in diagnosis of acute and chronic inflammatory conditions.

Name EIA Histamine RUO
Ref. IM2015
Method ELISA
Tests 12x8
Enzyme Alkaline phosphatase
Detection Absorbance at 405 nm
Sensitivity 0.5 nM
Incubation conditions 2 hrs/4°C,shaking (or 18 hrs/4°C) +30 min/RT;shaking
Calibrator range 0;1-100 nM
Sample volume (μL) 100
Sample form plasma;urine;whole blood;cell culture supernatants;other body liquids;extracts of solid biol. samples
Analyte info                      
Instructions for Use         
MSDS                             

 

 

Name EIA Histamine
Ref. IM2562
Method ELISA
Tests 12x8
Enzyme Alkaline phosphatase
Detection Absorbance at 405 nm
Sensitivity 0.5 nM
Incubation conditions 2 hrs/4°C,shaking (or 18 hrs/4°C) +30 min/RT;shaking
Calibrator range 0;1-100 nM
Sample volume (μL) 100
Sample form plasma;urine;whole blood;cell culture supernatants;other body liquids;extracts of solid biol. samples
Analyte info                      
Instructions for Use         
MSDS