Histamine, b-imidazolethylamine is an intercellular mediator exerting potent effects on various target tissues. Histamine is stored in large amounts in metachromatic granules of mast cells and basophils, and it is one of the major mediators of immediate hypersensitivity. It is released actively from mast cells and basophils when challenged with allergens to which the allergic patient is sensitized or with non-specific histamine releasers such as IgE or pharmacological agents. Histamine is also an important mediator in the central nervous system and the gastric mucosa. The determination of histamine can be used for the aid in diagnosis of acute and chronic inflammatory conditions.
Name |
EIA Histamine RUO |
Ref. |
IM2015 |
Method |
ELISA |
Tests |
12x8 |
Enzyme |
Alkaline phosphatase |
Detection |
Absorbance at 405 nm |
Sensitivity |
0.5 nM |
Incubation conditions |
2 hrs/4°C,shaking (or 18 hrs/4°C) +30 min/RT;shaking |
Calibrator range |
0;1-100 nM |
Sample volume (μL) |
100 |
Sample form |
plasma;urine;whole blood;cell culture supernatants;other body liquids;extracts of solid biol. samples |
Analyte info |
|
Instructions for Use |
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MSDS |
|
Name |
EIA Histamine |
Ref. |
IM2562 |
Method |
ELISA |
Tests |
12x8 |
Enzyme |
Alkaline phosphatase |
Detection |
Absorbance at 405 nm |
Sensitivity |
0.5 nM |
Incubation conditions |
2 hrs/4°C,shaking (or 18 hrs/4°C) +30 min/RT;shaking |
Calibrator range |
0;1-100 nM |
Sample volume (μL) |
100 |
Sample form |
plasma;urine;whole blood;cell culture supernatants;other body liquids;extracts of solid biol. samples |
Analyte info |
|
Instructions for Use |
|
MSDS |
|